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Huh7 and H1299 cells (1 10 4 per well) were sequentially treated with 1.5 M oligomycin, 1 M carbonyl cyanide 4-(trifluoromethoxy) phenylhydrazone and 1 M rotenoneantimycin A (both 0.5 M) (all from Sigma)

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Renal tubular cells have some of the highest glutathione concentrations in the human body a direct consequence of the kidney's filtration role, which exposes it to an enormous oxidative load every single day

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This observation may be attributable to the very low glutamate affinity of the L_180 class of NAD + -GDH (MSMEG_4699) [29]